Measurement of hexoses and pentoses, singly or in combination, with o-anisidine.
نویسندگان
چکیده
Hexoses and pentoses can be measured either singly or in combination, with an o-anisidine reagent. It has the advantages of a fastreactionrateand relativelylittle interference from hexoses (Amax = 660 nm) in the measurement of pentoses. A simple subtraction of absorbance attributable to glucose at the maximum for pentoses (465 nm) is sufficient for satisfactory pentose estimation in urine. The method has been automated, and resultscompare favorably with those from an automated procedure for the measurement of serum glucose inwhich hexokinase isused, and alsowiththose from a colorimetric aniline method for measurement of pentoses in urine. Interferences and procedural variables are discussed. Urinary pentose and hexose values for normal infants are reported.
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عنوان ژورنال:
- Clinical chemistry
دوره 19 6 شماره
صفحات -
تاریخ انتشار 1973